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Journal of Clinical Microbiology, May 2007, p. 1621-1623, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02145-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Impact of Competitive Inhibition and Sequence Variation upon the Sensitivity of Malaria PCR{triangledown}

Seweryn Bialasiewicz,1,2* David M. Whiley,1,2 Michael D. Nissen,1,2,3 and Theo P. Sloots1,2,3

Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Herston, Queensland, Australia,1 Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia,2 Microbiology Division, Queensland Health Pathology Service, Royal Brisbane Hospital Campus, Brisbane, Queensland, Australia3

Received 19 October 2006/ Returned for modification 29 November 2006/ Accepted 15 February 2007

Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

* Corresponding author. Mailing address: Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Building C28, Back Road, Royal Children's Hospital & Health Service District, Herston, Queensland, Australia 4029. Phone: 61-7-3636 1618. Fax: 61-7-3636 1401. E-mail: seweryn{at}uq.edu.au

{triangledown} Published ahead of print on 28 February 2007.

Journal of Clinical Microbiology, May 2007, p. 1621-1623, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02145-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shokoples, S. E., Ndao, M., Kowalewska-Grochowska, K., Yanow, S. K. (2009). Multiplexed Real-Time PCR Assay for Discrimination of Plasmodium Species with Improved Sensitivity for Mixed Infections. J. Clin. Microbiol. 47: 975-980 [Abstract] [Full Text]  


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