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Journal of Clinical Microbiology, June 2007, p. 1766-1770, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02162-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multicenter Laboratory Evaluation of the MB/BacT Mycobacterium Detection System and the BACTEC MGIT 960 System in Comparison with the BACTEC 460TB System for Susceptibility Testing of Mycobacterium tuberculosis{triangledown}

Montserrat Garrigó,1,2 Lina Marcela Aragón,1 Fernando Alcaide,3 Sonia Borrell,4 Eugenia Cardeñosa,3 Juan José Galán,5 Julián Gonzalez-Martín,4 Nuria Martin-Casabona,5 Carmen Moreno,1 Margarita Salvado,6 and Pere Coll1,2*

Servei de Microbiología, Hospital de la Santa Creu i Sant Pau,1 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona,2 Hospital Universitari de Bellvitge,3 Departamento de Microbiología, Hospital Clínic-IDIBAPS,4 Hospital Vall d'Hebron,5 Hospital del Mar, Barcelona, Spain6

Received 23 October 2006/ Returned for modification 10 January 2007/ Accepted 11 April 2007

In this multicenter study, the reliability of two nonradiometric, fully automated systems, the MB/BacT and BACTEC MGIT 960 systems, for testing the susceptibilities of 82 Mycobacterium tuberculosis strains to isoniazid, rifampin, ethambutol, and streptomycin was evaluated in comparison with the radiometric BACTEC 460TB system. The arbitration of discrepant results was done by the reanalysis of the strain, the determination of the MIC, and the molecular characterization of some resistance determinants. The overall level of agreement with BACTEC 460TB results was 96% with the MB/BacT test and 97.2% with the BACTEC MGIT 960 system. With both methods, the level of agreement with BACTEC 460TB results was 96.3% for isoniazid, 98.8% for rifampin, and 98.8% for ethambutol. The level of agreement for streptomycin was 90.2% with MB/BacT and 97.5% with BACTEC MGIT 960. Overall, there were 11 very major errors and 2 major errors with the MB/BacT method and 5 very major errors and 2 major errors with the BACTEC MGIT 960 system. In general, the MB/BacT and BACTEC MGIT 960 systems showed good performance for susceptibility testing with first-line antituberculosis drugs.

* Corresponding author. Mailing address: Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Av. Sant Antoni Ma Claret, 167, 08025 Barcelona, Spain. Phone: 34 93 2919069. Fax: 3493 2919070. E-mail: pcoll{at}santpau.es

{triangledown} Published ahead of print on 18 April 2007.

Journal of Clinical Microbiology, June 2007, p. 1766-1770, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02162-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Krishnan, M. Y., Manning, E. J. B., Collins, M. T. (2009). Comparison of three methods for susceptibility testing of Mycobacterium avium subsp. paratuberculosis to 11 antimicrobial drugs. J Antimicrob Chemother 64: 310-316 [Abstract] [Full Text]  


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