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Journal of Clinical Microbiology, June 2007, p. 1904-1911, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02500-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparative Genomics of Canadian Epidemic Lineages of Methicillin-Resistant Staphylococcus aureus{triangledown} ,{dagger}

Sara Christianson,1,2 George R. Golding,1,2 Jennifer Campbell,1 the Canadian Nosocomial Infection Surveillance Program {ddagger} Michael R. Mulvey,1,2*

National Microbiology Laboratory, 1015 Arlington Ave., Winnipeg, Manitoba R3E 3R2, Canada,1 University of Manitoba, 730 William Ave, Winnipeg, Manitoba R3E 0W3, Canada2

Received 13 December 2006/ Returned for modification 20 January 2007/ Accepted 30 March 2007

Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that has disseminated throughout Canadian hospitals and communities. Pulsed-field gel electrophoresis of over 9,300 MRSA isolates obtained from the Canadian Nosocomial Infection Surveillance Program has identified 10 epidemic strain types in Canada (CMRSA1 to CMRSA10). In an attempt to determine specific genetic factors that have contributed to their high prevalence in community and/or hospital settings, the genomic content of representative isolates for each of the 10 Canadian epidemic types was compared using comparative genomic hybridizations. Comparison of the community-associated Canadian epidemic isolates (CMRSA7 and CMRSA10) with the hospital-associated Canadian epidemic isolates revealed one open reading frame (ORF) (SACOL0046) encoding a putative protein belonging to a metallo-beta-lactamase family, which was present only in the community-associated Canadian epidemic isolates. A more restricted comparison involving only the most common hospital-associated Canadian epidemic isolates (CMRSA1 and CMRSA2) with the community-associated Canadian epidemic isolates did reveal additional factors that might be contributing to their prevalence in the community and hospital settings, which included ORFs encoding potential virulence factors involved in capsular biosynthesis, serine proteases, epidermin, adhesion factors, regulatory functions, leukotoxins, and exotoxins.

* Corresponding author. Mailing address: National Microbiology Laboratory, 1015 Arlington Ave., Winnipeg, Manitoba R3E 3R2, Canada. Phone: (204) 789-2133. Fax: (204) 789-5020. E-mail: Michael_Mulvey{at}phac-aspc.gc.ca

{triangledown} Published ahead of print on 11 April 2007.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} Members of the Canadian Nosocomial Infection Surveillance Program (CNISP) are as follows: David Boyd, National Microbiology Laboratory, Public Health Agency of Canada; Elizabeth Bryce, Vancouver General Hospital, Vancouver, British Columbia; John Conly, Foothills Medical Centre, Calgary, Alberta; Gordon Dow, The Moncton Hospital, Moncton, New Brunswick; John Embil, Health Sciences Centre, Winnipeg, Manitoba; Joanne Embree, Health Sciences Centre, Winnipeg, Manitoba; Charles Frenette, Hôpital Charles LeMoyne, Longueil, Quebec; Michael Gardam, University Health Network, Toronto, Ontario; Denise Gravel, Centre for Infectious Disease Prevention and Control, Public Health Agency of Canada; Elizabeth Henderson, Peter Lougheed Centre, Calgary, Alberta; James Hutchinson, Health Sciences Centre, St. John's, Newfoundland; Michael John, London Health Sciences Centre, London, Ontario; Lynn Johnston, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia; Pamela Kibsey, Victoria General Hospital, Victoria, British Columbia; Joanne Langley, I. W. K. Health Science Centre, Halifax, Nova Scotia; Mark Loeb, Hamilton Health Sciences Corporation, Hamilton, Ontario; Anne Matlow, Hospital for Sick Children, Toronto, Ontario; Allison McGeer, Mount Sinai Hospital, Toronto, Ontario; Sophie Michaud, CHUS-Hôpital Fleurimont, Sherbrooke, Quebec; Mark Miller, SMBD-Jewish General Hospital, Montreal, Quebec; Dorothy Moore, Montreal Children's Hospital, Montreal, Quebec; Michael Mulvey, National Microbiology Laboratory, Public Health Agency of Canada; Marianna Ofner-Agostini, Centre for Infectious Disease Prevention and Control, Public Health Agency of Canada; Shirley Paton, Centre for Infectious Disease Prevention and Control, Public Health Agency of Canada; Virginia Roth, The Ottawa Hospital, Ottawa, Ontario; Andrew Simor, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario; Jacob Stegenga, Centre for Infectious Disease Prevention and Control, Public Health Agency of Canada; Tammy Stuart, Canadian Field Epidemiology Program, Public Health Agency of Canada; Kathryn Suh, Children's Hospital of Eastern Ontario, Ottawa, Ontario; Geoffrey Taylor, University of Alberta Hospital, Edmonton, Alberta; Eva Thomas, Children's and Women's Health Center, Vancouver, British Columbia; Nathalie Turgeon, Hôtel-Dieu de Québec du CHUQ, Quebec; Mary Vearncombe, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario; Joseph Vayalumkal, Canadian Field Epidemiology Program, Public Health Agency of Canada; Karl Weiss, Maisonneuve-Rosemont Hospital, Montreal, Quebec; Alice Wong, Royal University Hospital, Saskatoon, Saskatchewan; and Dick Zoutman, Kingston General Hospital, Kingston, Ontario.

Journal of Clinical Microbiology, June 2007, p. 1904-1911, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02500-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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