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Journal of Clinical Microbiology, June 2007, p. 1974-1977, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02087-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Etest and Disk Diffusion Methods Compared with Broth Microdilution Antifungal Susceptibility Testing of Clinical Isolates of Candida spp. against Posaconazole{triangledown}

Daniel J. Diekema,1,2* Shawn A. Messer,1 Richard J. Hollis,1 Linda B. Boyken,1 Shailesh Tendolkar,1 Jennifer Kroeger,1 and Michael A. Pfaller1,3

Departments of Pathology,1 Epidemiology,2 Internal Medicine, Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, Iowa City, Iowa 522423

Received 10 October 2006/ Returned for modification 27 December 2006/ Accepted 5 February 2007

We performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical isolates of Candida spp. against posaconazole. By using provisional breakpoints for comparison purposes only, the categorical agreement between the agar-based methods and broth microdilution results ranged from 93 to 98%, with <1% very major errors. The essential agreement (within 2 well dilutions) between the Etest and broth microdilution methods was 94%. These agar-based methods hold promise as simple and reliable methods for determination of the posaconzole susceptibilities of Candida spp.

* Corresponding author. Mailing address: Department of Pathology, Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242. Phone: (319) 356-8615. Fax: (319) 356-4916. E-mail: daniel-diekema{at}uiowa.edu

{triangledown} Published ahead of print on 14 February 2007.

Journal of Clinical Microbiology, June 2007, p. 1974-1977, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02087-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Morris, M. I. (2009). Posaconazole: A new oral antifungal agent with an expanded spectrum of activity. Am J Health Syst Pharm 66: 225-236 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Boyken, L., Tendolkar, S., Hollis, R. J., Diekema, D. J. (2008). Selection of a Surrogate Agent (Fluconazole or Voriconazole) for Initial Susceptibility Testing of Posaconazole against Candida spp.: Results from a Global Antifungal Surveillance Program. J. Clin. Microbiol. 46: 551-559 [Abstract] [Full Text]  
  • Canton, E., Peman, J., Espinel-Ingroff, A., Martin-Mazuelos, E., Carrillo-Munoz, A., Martinez, J. P. (2008). Comparison of disc diffusion assay with the CLSI reference method (M27-A2) for testing in vitro posaconazole activity against common and uncommon yeasts. J Antimicrob Chemother 61: 135-138 [Abstract] [Full Text]  


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