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Journal of Clinical Microbiology, June 2007, p. 1978-1980, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00563-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of an Emerging Pathogen, Mycobacterium massiliense, by rpoB Sequencing of Clinical Isolates Collected in the United States{triangledown}

Keith E. Simmon,1 June I. Pounder,1 John N. Greene,2 Frank Walsh,3 Clint M. Anderson,4 Samuel Cohen,4 and Cathy A. Petti1,4,5*

Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah,1 H. Lee Moffitt Cancer and Research Institute, Tampa, Florida,2 Department of Medicine, University of South Florida College of Medicine, Tampa, Florida,3 ARUP Laboratories, Salt Lake City, Utah,4 Departments of Medicine and Pathology, University of Utah School of Medicine, Salt Lake City, Utah5

Received 13 March 2007/ Accepted 26 March 2007

Mycobacterium massiliense is a rapidly growing mycobacterium that is indistinguishable from Mycobacterium chelonae/M. abscessus by partial 16S rRNA gene sequencing. We sequenced rpoB, sodA, and hsp65 genes from isolates previously identified as being M. chelonae/M. abscessus and identified M. massiliense from isolates from two patients with invasive disease representing the first reported cases in the United States.

* Corresponding author. Mailing address: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787. Fax: (801) 584-5207. E-mail: cathy.petti{at}aruplab.com

{triangledown} Published ahead of print on 4 April 2007.

Journal of Clinical Microbiology, June 2007, p. 1978-1980, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.00563-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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