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Journal of Clinical Microbiology, June 2007, p. 2037-2039, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02432-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development of a Serological Assay Based on a Synthetic Peptide Selected from the VP0 Capsid Protein for Detection of Human Parechoviruses{triangledown}

Yacine Abed,1* Dana Wolf,2 Ron Dagan,3 and Guy Boivin1*

Research Center in Infectious Diseases, Centre Hospitalier Universitaire de Québec and Laval University, Québec City, Québec, Canada,1 Department of Clinical Microbiology and Infectious Diseases and of Virology, Hadassah University Hospital and Medical School, Jerusalem, Israel,2 Pediatric Infectious Diseases Unit, Soroka University Medical Center, Ben Gurion University of the Negev, Beer-Sheva, Israel3

Received 4 December 2006/ Returned for modification 16 January 2007/ Accepted 7 April 2007

A serological enzyme-linked immunosorbent assay was developed using a synthetic peptide from the VP0 protein of human parechoviruses (HPeVs). Seroprevalence for HPeVs was 70% in children of ≤5 years of age and 95% in adults. For children from whom serial sera were sampled, seropositivity increased from 22% to 88% between 2 and 24 months of age.

* Corresponding author. Mailing address: Research Center in Infectious Diseases, CHUL, Room RC-709, 2705 Blvd. Laurier, Sainte-Foy, QC, Canada G1V 4G2. Phone for Guy Boivin: (418) 654-2705. Fax: (418) 654-2715. E-mail: Guy.Boivin{at}crchul.ulaval.ca. Phone for Yacine Abed: (418) 654-2705. Fax: (418) 654-2715. E-mail: Yacine.Abed{at}crchul.ulaval.ca

{triangledown} Published ahead of print on 18 April 2007.

Journal of Clinical Microbiology, June 2007, p. 2037-2039, Vol. 45, No. 6
0095-1137/07/$08.00+0     doi:10.1128/JCM.02432-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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