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Journal of Clinical Microbiology, July 2007, p. 2151-2155, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.02308-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Various Blood Compartments and Reporting Units for the Detection and Quantification of Epstein-Barr Virus in Peripheral Blood{triangledown}

H. Hakim,1 C. Gibson,2 J. Pan,3 K. Srivastava,3 Z. Gu,2 M. J. Bankowski,4 and R. T. Hayden2*

Departments of Infectious Diseases,1 Pathology,2 Biostatistics, St. Jude Children's Research Hospital, Memphis, Tennessee,3 Department of Microbiology, Diagnostic Laboratory Services, Inc., and the Queens and Kuakini Health Systems, Honolulu, Hawaii4

Received 14 November 2006/ Returned for modification 14 February 2007/ Accepted 1 May 2007

Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-time PCR (QRT-PCR), we evaluated EBV in whole blood (WB), peripheral blood mononuclear cells (PBMC), and plasma in 249 samples from 122 patients. In WB and PBMC, results were reported both in viral copies/ml and in copies/µg of total DNA. Trendings of quantitative values over time among the different sample types were compared. The sensitivities of QRT-PCR using WB and that using PBMC did not differ significantly (P = 0.33), and both were more sensitive than plasma alone (P < 0.0001). EBV viral load results from WB and PBMC paired sample types also showed a significant correlation (P < 0.05), as did results reported in copies/ml and copies/µg DNA for both WB and PBMC (R2 > 0.93). EBV viral loads detected using WB and PBMC trended very closely for the few patients who had multiple positive samples available for analysis. WB and PBMC show comparable sensitivities and a close quantitative correlation when assayed for EBV by QRT-PCR. The close correlation between copies/ml and copies/µg DNA also suggests that normalization to cell number or genomic DNA in cellular specimens may not be necessary.

* Corresponding author. Mailing address: St. Jude Children's Research Hospital, Department of Pathology, 332 North Lauderdale Street, Memphis, TN 38105. Phone: (901) 495-3300. Fax: (901) 495-3100. E-mail: randall.hayden{at}stjude.org

{triangledown} Published ahead of print on 9 May 2007.

Journal of Clinical Microbiology, July 2007, p. 2151-2155, Vol. 45, No. 7
0095-1137/07/$08.00+0     doi:10.1128/JCM.02308-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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