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Journal of Clinical Microbiology, August 2007, p. 2380-2384, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00776-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Phoenix and VITEK 2 Extended-Spectrum-ß-Lactamase Detection Tests for Analysis of Escherichia coli and Klebsiella Isolates with Well-Characterized ß-Lactamases{triangledown}

Kenneth S. Thomson,1* Nancy E. Cornish,2 Seong G. Hong,1 Kim Hemrick,2 Christian Herdt,2 and Ellen S. Moland1

Center for Research in Antiinfectives and Biotechnology (CRAB), Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178,1 Microbiology Laboratory, Methodist Hospital, Omaha, Nebraska 681142

Received 11 April 2007/ Returned for modification 31 May 2007/ Accepted 14 June 2007

The VITEK 2 and Phoenix extended-spectrum ß-lactamase (ESBL) detection systems, which comprise confirmatory tests and expert systems, were evaluated for their ability to discriminate between 102 well-characterized strains of ESBL-positive or -negative Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca. At least 38 distinct ESBLs were included. The strains were chosen to include some known to cause false-positive and false-negative CLSI ESBL confirmatory test results. Therefore, enzyme characterizations, rather than CLSI tests, were the reference methods for the Phoenix and VITEK 2 evaluations. A third arm of the study was conducted with the Phoenix test using two normally inactive expert rules intended to enhance ESBL detection, in addition to using the currently available software. The Phoenix ESBL confirmatory test and unmodified expert system exhibited 96% sensitivity and 81% specificity for ESBL detection. Activation of the two additional rules increased sensitivity to 99% but reduced the specificity to 58%. The VITEK 2 ESBL confirmatory test exhibited 91% sensitivity, which was reduced to 89% sensitivity by its expert system, while its specificity was 85%. Many of the expert system interpretations of both instruments were helpful, but some were suboptimal. The VITEK 2 expert system was potentially more frustrating because it provided more inconclusive interpretations of the results. Considering the high degree of diagnostic difficulty posed by the strains, both ESBL confirmatory tests were highly sensitive. The expert systems of both instruments require modification to update and enhance their utility.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178. Phone: (402) 280-4096. Fax: (402) 280-1875. E-mail: kstaac{at}creighton.edu

{triangledown} Published ahead of print on 27 June 2007.

Journal of Clinical Microbiology, August 2007, p. 2380-2384, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00776-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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