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Journal of Clinical Microbiology, August 2007, p. 2452-2459, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00699-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing{triangledown}

Chi-Yang Lin,1 Yee-Chun Chen,2 Hsiu-Jung Lo,3 Kuo-Wei Chen,1 and Shu-Ying Li1*

Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei, Taiwan,1 Division of Infectious Diseases, Department of Internal Medicine, National Taiwan University Hospital, and Department of Medicine, National Taiwan University College of Medicine, Taipei, Taiwan,2 Division of Clinical Research, National Health Research Institutes, Miaoli County, Taiwan3

Received 30 March 2007/ Returned for modification 24 April 2007/ Accepted 29 May 2007

In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.

* Corresponding author. Mailing address: Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, No. 161, Kun-Yang Street, 11561 Taipei, Taiwan. Phone: 886-2-26531388. Fax: 886-2-26513572. E-mail: syl{at}cdc.gov.tw

{triangledown} Published ahead of print on 6 June 2007.

Journal of Clinical Microbiology, August 2007, p. 2452-2459, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00699-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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