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Journal of Clinical Microbiology, August 2007, p. 2498-2507, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00038-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Characterization and Distribution of Genes Encoding Members of the Type III Effector NleA Family among Pathogenic Escherichia coli Strains{triangledown}

Kristina Creuzburg and Herbert Schmidt*

Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, 70599 Stuttgart, Germany

Received 5 January 2007/ Returned for modification 24 February 2007/ Accepted 23 May 2007

In this study, we investigated the occurrence of the previously described gene nleA4795 and variants of nleA, putatively encoding non-locus-of-enterocyte-effacement-encoded type III effector proteins with functions that are unknown. nleA variants were detected in 150 out of 170 enteropathogenic Escherichia coli strains and enterohemorrhagic E. coli strains, two of them being eae negative. Besides the known variants nleA4795, Z6024, and the espI-like gene, 11 novel nleA variants with different lengths and sequence identities at the deduced amino acid level (between 71% and 96%) have been identified. Whereas most of the serogroups associated with more severe disease were quite homogenous with respect to the presence of a particular nleA variant, other serogroups were not. Moreover, Southern blot hybridization revealed that certain strains carry two copies of nleA in their chromosome, frequently encoding different variants. In most cases, the open reading frame of one of the copies was disrupted, usually by an insertion element. Furthermore, transmission of the type III effector-encoding gene could be shown by transduction of nleA-carrying bacteriophages to a laboratory E. coli strain.

* Corresponding author. Mailing address: Department of Food Microbiology, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstraße 28, 70599 Stuttgart, Germany. Phone: 49-711/459-22305. Fax: 49-711/459-24199. E-mail: hschmidt{at}uni-hohenheim.de

{triangledown} Published ahead of print on 6 June 2007.

Journal of Clinical Microbiology, August 2007, p. 2498-2507, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00038-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Slanec, T., Fruth, A., Creuzburg, K., Schmidt, H. (2009). Molecular Analysis of Virulence Profiles and Shiga Toxin Genes in Food-Borne Shiga Toxin-Producing Escherichia coli. Appl. Environ. Microbiol. 75: 6187-6197 [Abstract] [Full Text]  
  • Garcia-Angulo, V. A., Deng, W., Thomas, N. A., Finlay, B. B., Puente, J. L. (2008). Regulation of Expression and Secretion of NleH, a New Non-Locus of Enterocyte Effacement-Encoded Effector in Citrobacter rodentium. J. Bacteriol. 190: 2388-2399 [Abstract] [Full Text]  


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