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Journal of Clinical Microbiology, August 2007, p. 2691-2694, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00085-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Delayed Processing of Blood Samples Influences Time to Positivity of Blood Cultures and Results of Gram Stain-Acridine Orange Leukocyte Cytospin Test{triangledown}

I. Schwetz,1 G. Hinrichs,1 E. C. Reisinger,2 G. J. Krejs,3 H. Olschewski,1 and R. Krause1*

Division of Infectious Diseases, Department of Pulmonology, Medical University of Graz, Graz, Austria,1 Division of Infectious Diseases and Tropical Medicine, University of Rostock, Rostock, Germany,2 Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria3

Received 11 January 2007/ Returned for modification 1 May 2007/ Accepted 17 May 2007

We investigated in vitro whether storage of blood samples influences the time to positivity used for the calculation of the differential time to positivity (DTP) and the results of the Gram stain-acridine orange leukocyte Cytospin (AOLC) test. A 24-hour storage of blood samples at room temperature may lead to false-negative DTP and false-positive Gram stain-AOLC test results, whereas storage at 4°C does not.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Pulmonology, Medical University of Graz, Auenbruggerplatz 20, A-8036 Graz, Austria. Phone: (43-316) 385-2183. Fax: (43-316) 385-3578. E-mail: robert.krause{at}meduni-graz.at

{triangledown} Published ahead of print on 30 May 2007.

Journal of Clinical Microbiology, August 2007, p. 2691-2694, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00085-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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