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Journal of Clinical Microbiology, August 2007, p. 2695-2697, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00562-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Field Evaluation of a Rota- and Adenovirus Immunochromatographic Assay Using Stool Samples from Children with Acute Diarrhea in Ghana{triangledown}

Thomas Weitzel,1* Klaus Reither,1,2 Frank P. Mockenhaupt,1 Klaus Stark,3 Ralf Ignatius,4 Eiman Saad,1 Andrew Seidu-Korkor,5 Ulrich Bienzle,1 and Eckart Schreier6

Institute of Tropical Medicine and International Health, Charité, University Medicine Berlin, Berlin, Germany,1 Department of Infectious Diseases and Tropical Medicine, University of Munich, Munich, Germany,2 Department of Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany,3 Institute of Microbiology and Hygiene, Charité, University Medicine Berlin, Berlin, Germany,4 Regional Health Administration, Ministry of Health, Tamale, Ghana,5 Department of Molecular Epidemiology of Viral Pathogens, Robert Koch Institute, Berlin, Germany6

Received 13 March 2007/ Returned for modification 4 June 2007/ Accepted 12 June 2007

We evaluated the Rida Quick rotavirus/adenovirus Combi rapid immunochromatographic test (ICT) under field conditions with Ghanaian children with acute diarrhea. Compared to PCR results, sensitivities and specificities were 75% and 95% for rotavirus and 22% and 84% for adenovirus. In resource-poor settings, ICTs may help to overcome difficulties in the diagnosis of rotavirus infection.

* Corresponding author. Mailing address: Institute of Tropical Medicine and International Health, Charité, University Medicine Berlin, Spandauer Damm 130, D-14050 Berlin, Germany. Phone: 49 30 30116 816. Fax: 49 30 30116 888. E-mail: thomas.weitzel{at}charite.de

{triangledown} Published ahead of print on 27 June 2007.

Journal of Clinical Microbiology, August 2007, p. 2695-2697, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00562-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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