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Journal of Clinical Microbiology, August 2007, p. 2726-2730, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00321-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real-Time PCR Approach{triangledown}

Roger Dumke,* Nicol Schurwanz, Matthias Lenz, Markus Schuppler,{dagger} Christian Lück, and Enno Jacobs

Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany

Received 9 February 2007/ Returned for modification 22 March 2007/ Accepted 18 May 2007

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

* Corresponding author. Mailing address: Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. Phone: 49 351 4586577. Fax: 49 351 4586310. E-mail: roger.dumke{at}mailbox.tu-dresden.de

{triangledown} Published ahead of print on 30 May 2007.

{dagger} Present address: Swiss Federal Institute of Technology Zürich, Institute of Food Science and Nutrition, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland.

Journal of Clinical Microbiology, August 2007, p. 2726-2730, Vol. 45, No. 8
0095-1137/07/$08.00+0     doi:10.1128/JCM.00321-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Touati, A., Benard, A., Hassen, A. B., Bebear, C. M., Pereyre, S. (2009). Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens. J. Clin. Microbiol. 47: 2269-2271 [Abstract] [Full Text]  
  • Dumke, R., Jacobs, E. (2009). Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae. J. Clin. Microbiol. 47: 441-444 [Abstract] [Full Text]  


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