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Journal of Clinical Microbiology, September 2007, p. 2943-2950, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.02107-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Assessment of Use of Microsatellite Polymorphism Analysis for Improving Spatial Distribution Tracking of Echinococcus multilocularis{triangledown}

J. Knapp,1,2* J. M. Bart,2 M. L. Glowatzki,3 A. Ito,4 S. Gerard,2 S. Maillard,2 R. Piarroux,2 and B. Gottstein1

Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland,1 Parasitology Department, SERF Team (Equipe Santé Environnement Rural Franche-Comté), Faculty of Medicine, University of Franche-Comté, Besançon, France,2 Department of Clinical Research, Vetsuisse Faculty, University of Bern, Bern, Switzerland,3 Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido, Japan4

Received 13 October 2006/ Returned for modification 1 January 2007/ Accepted 6 July 2007

Alveolar echinococcosis (AE)—caused by the cestode Echinococcus multilocularis—is a severe zoonotic disease found in temperate and arctic regions of the northern hemisphere. Even though the transmission patterns observed in different geographical areas are heterogeneous, the nuclear and mitochondrial targets usually used for the genotyping of E. multilocularis have shown only a marked genetic homogeneity in this species. We used microsatellite sequences, because of their high typing resolution, to explore the genetic diversity of E. multilocularis. Four microsatellite targets (EmsJ, EmsK, and EmsB, which were designed in our laboratory, and NAK1, selected from the literature) were tested on a panel of 76 E. multilocularis samples (larval and adult stages) obtained from Alaska, Canada, Europe, and Asia. Genetic diversity for each target was assessed by size polymorphism analysis. With the EmsJ and EmsK targets, two alleles were found for each locus, yielding two and three genotypes, respectively, discriminating European isolates from the other groups. With NAK1, five alleles were found, yielding seven genotypes, including those specific to Tibetan and Alaskan isolates. The EmsB target, a tandem repeated multilocus microsatellite, found 17 alleles showing a complex pattern. Hierarchical clustering analyses were performed with the EmsB findings, and 29 genotypes were identified. Due to its higher genetic polymorphism, EmsB exhibited a higher discriminatory power than the other targets. The complex EmsB pattern was able to discriminate isolates on a regional and sectoral level, while avoiding overdistinction. EmsB will be used to assess the putative emergence of E. multilocularis in Europe.

* Corresponding author. Mailing address: Parasitology Department, SERF Team, Faculty of Medicine, University of Franche-Comté, 19 rue Ambroise Paré, 25000 Besançon, France. Phone: 00 33 3 63 08 22 36. Fax: 00 33 3 63 08 22 32. E-mail: jenny.knapp{at}univ-fcomte.fr

{triangledown} Published ahead of print on 18 July 2007.

Journal of Clinical Microbiology, September 2007, p. 2943-2950, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.02107-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Maillard, S., Gottstein, B., Haag, K. L., Ma, S., Colovic, I., Benchikh-Elfegoun, M. C., Knapp, J., Piarroux, R. (2009). The EmsB Tandemly Repeated Multilocus Microsatellite: a New Tool To Investigate Genetic Diversity of Echinococcus granulosus Sensu Lato. J. Clin. Microbiol. 47: 3608-3616 [Abstract] [Full Text]  


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