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Journal of Clinical Microbiology, September 2007, p. 3065-3067, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00891-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Diversity of Mutations in the atpC Gene Coding for the c Subunit of F0F1 ATPase in Clinical Isolates of Optochin-Resistant Streptococcus pneumoniae from Brazil{triangledown}

Cícero A. Dias,1,3 Grasiela Agnes,1 Ana Paula G. Frazzon,1 Filipe D. Kruger,1 Pedro A. d'Azevedo,1 Maria da Glória S. Carvalho,2 Richard R. Facklam,2 and Lúcia M. Teixeira3*

Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil,1 Centers for Disease Control and Prevention, Atlanta, Georgia 30333,2 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil3

Received 29 April 2007/ Returned for modification 3 June 2007/ Accepted 2 July 2007

We report the characteristics of four optochin-resistant (Optr) Streptococcus pneumoniae isolates from Brazil. All four Optr isolates presented mutations in the nucleotide sequence coding for the c subunit of F0F1 ATPase. Two isolates showed mutations in codons 23 (leading to the deduced amino acid substitution isoleucine instead of alanine) and 49 (serine instead of alanine, a novel type of mutation detected at this position), respectively. Two additional novel mutations, both located in codon 45, were detected in the other two isolates, corresponding to leucine or valine (instead of phenylalanine). The data indicate that three previously unrecognized alterations were detected in the atpC gene of S. pneumoniae and that Opt resistance among Brazilian pneumococcal isolates is not related to a specific pneumococcal serotype, antimicrobial-resistance profile, or clonal group.

* Corresponding author. Mailing address: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Cidade Universitária, Rio de Janeiro, RJ 21941-590, Brazil. Phone: 55 21 2260 4193. Fax: 55 21 2560 8344. E-mail: lmt2{at}micro.ufrj.br

{triangledown} Published ahead of print on 11 July 2007.

Journal of Clinical Microbiology, September 2007, p. 3065-3067, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00891-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cortes, P. R., Orio, A. G. A., Regueira, M., Pinas, G. E., Echenique, J. (2008). Characterization of In Vitro-Generated and Clinical Optochin-Resistant Strains of Streptococcus pneumoniae Isolated from Argentina. J. Clin. Microbiol. 46: 1930-1934 [Abstract] [Full Text]  
  • Nunes, S., Sa-Leao, R., de Lencastre, H. (2008). Optochin Resistance among Streptococcus pneumoniae Strains Colonizing Healthy Children in Portugal. J. Clin. Microbiol. 46: 321-324 [Abstract] [Full Text]  


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