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Journal of Clinical Microbiology, September 2007, p. 3072-3076, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.01131-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Quantitative PCR and Culture Methods To Characterize Ecological Flux in Bacterial Biofilms{triangledown}

F. Dalwai,* D. A. Spratt, and J. Pratten

Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom

Received 6 June 2007/ Accepted 21 June 2007

An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.

* Corresponding author. Mailing address: Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom. Phone: 0044 207 915 1016. Fax: 0044 207 915 1127. E-mail: f.dalwai{at}eastman.ucl.ac.uk

{triangledown} Published ahead of print on 27 June 2007.

Journal of Clinical Microbiology, September 2007, p. 3072-3076, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.01131-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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