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Journal of Clinical Microbiology, September 2007, p. 3089-3094, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00858-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens{triangledown} ,{dagger}

N. Guimarães,1,2 N. F. Azevedo,1,3 C. Figueiredo,2,4 C. W. Keevil,3 and M. J. Vieira1*

IBB (Institute for Biotechnology and Bioengineering), Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga,1 IPATIMUP (Institute of Molecular Pathology and Immunology of the University of Porto),2 Medical Faculty of Porto, Porto, Portugal,4 School of Biological Sciences, University of Southampton, Southampton, United Kingdom3

Received 24 April 2007/ Accepted 31 May 2007

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

* Corresponding author. Mailing address: Centro de Engenharia Biológica, Universidade do Minho, 4710-057 Braga, Portugal. Phone: 351 253 604411. Fax: 351 253 678986. E-mail: mjv{at}deb.uminho.pt

{triangledown} Published ahead of print on 3 July 2007.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, September 2007, p. 3089-3094, Vol. 45, No. 9
0095-1137/07/$08.00+0     doi:10.1128/JCM.00858-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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