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Journal of Clinical Microbiology, January 2008, p. 314-316, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01313-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Specimen Dilution Improves Sensitivity of the Amplified Mycobacterium tuberculosis Direct Test for Smear Microscopy-Positive Respiratory Specimens{triangledown}

Renata L. Guerra,1 James F. Baker,2 Roya Alborz,2 Derek T. Armstrong,2 Julia A. Kiehlbauch,2 Marcus B. Conde,3 Susan E. Dorman,1* and Nancy M. Hooper2

Johns Hopkins University School of Medicine,1 Maryland Department of Health and Mental Hygiene, Baltimore, Maryland,2 Instituto de Doenças do Tórax-Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil3

Received 29 June 2007/ Returned for modification 9 September 2007/ Accepted 21 October 2007

Specimen dilution has been proposed as a strategy to minimize amplified Mycobacterium tuberculosis direct (MTD) test inhibition (N. Pollock, J. Westerling, and A. Sloutsky, Am. J. Clin. Pathol. 126:142-147, 2006; A. Sloutsky, L. L. Han, and B. G. Werner, J. Clin. Microbiol. 42:1547-1551, 2004). We evaluated the impact of respiratory specimen dilution on MTD test accuracy in a public health laboratory. The difference in MTD test sensitivity between the dilution and conventional methods was 15.9% (P = 0.001) for smear microscopy-positive specimens and –3.6% (P = 0.38) for smear microscopy-negative specimens.

* Corresponding author. Mailing address: Johns Hopkins University Center for Tuberculosis Research, 1550 Orleans St., Room 1M-06, Baltimore, MD 21231. Phone: (410) 502-2717. Fax: (410) 955-0740. E-mail: DSUSAN1{at}JHMI.EDU

{triangledown} Published ahead of print on 31 October 2007.

Journal of Clinical Microbiology, January 2008, p. 314-316, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01313-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, J. Y., Ferraro, M. J., Branda, J. A. (2009). False-Negative Results Obtained with the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test Caused by Unrecognized Inhibition of the Amplification Reaction. J. Clin. Microbiol. 47: 2995-2997 [Abstract] [Full Text]  
  • Guerra, R. L., Hooper, N. M., Baker, J. F., Alborz, R., Armstrong, D. T., Kiehlbauch, J. A., Conde, M. B., Dorman, S. E. (2008). Cost-Effectiveness of Different Strategies for Amplified Mycobacterium tuberculosis Direct Testing for Cases of Pulmonary Tuberculosis. J. Clin. Microbiol. 46: 3811-3812 [Abstract] [Full Text]  


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