This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fenner, L.
Right arrow Articles by Frei, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fenner, L.
Right arrow Articles by Frei, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2008, p. 328-330, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01503-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid and Reliable Diagnostic Algorithm for Detection of Clostridium difficile{triangledown}

Lukas Fenner,1 Andreas F. Widmer,2 Gisela Goy,1 Sonja Rudin,1 and Reno Frei1*

Microbiology Laboratory,1 Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Petersgraben 4, CH-4031 Basel, Switzerland2

Received 26 July 2007/ Returned for modification 20 September 2007/ Accepted 2 November 2007

We evaluated a two-step algorithm for detection of Clostridium difficile in 1,468 stool specimens. First, specimens were screened by an immunoassay for C. difficile glutamate dehydrogenase antigen (C.DIFF CHEK-60). Second, screen-positive specimens underwent toxin testing by a rapid toxin A/B assay (TOX A/B QUIK CHEK); toxin-negative specimens were subjected to stool culture. This algorithm allowed final results for 92% of specimens with a turnaround time of 4 h.

* Corresponding author. Mailing address: Microbiology Laboratory, University Hospital Basel, Petersgraben 4, CH-4031 Basel, Switzerland. Phone: 41 61 265 42 44. Fax: 41 61 265 53 55. E-mail: rfrei{at}uhbs.ch

{triangledown} Published ahead of print on 21 November 2007.

Journal of Clinical Microbiology, January 2008, p. 328-330, Vol. 46, No. 1
0095-1137/08/$08.00+0     doi:10.1128/JCM.01503-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, H., Weintraub, A., Fang, H., Nord, C. E. (2009). Comparison of a Commercial Multiplex Real-Time PCR to the Cell Cytotoxicity Neutralization Assay for Diagnosis of Clostridium difficile Infections. J. Clin. Microbiol. 47: 3729-3731 [Abstract] [Full Text]  
  • Eastwood, K., Else, P., Charlett, A., Wilcox, M. (2009). Comparison of Nine Commercially Available Clostridium difficile Toxin Detection Assays, a Real-Time PCR Assay for C. difficile tcdB, and a Glutamate Dehydrogenase Detection Assay to Cytotoxin Testing and Cytotoxigenic Culture Methods. J. Clin. Microbiol. 47: 3211-3217 [Abstract] [Full Text]  
  • Williams, O. M., Spencer, R. C. (2009). The management of Clostridium difficile infection. Br Med Bull 91: 87-110 [Abstract] [Full Text]  
  • Bloedt, K., Riecker, M., Poppert, S., Wellinghausen, N. (2009). Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples. J Med Microbiol 58: 874-877 [Abstract] [Full Text]  
  • Misteli, H., Weber, W. P., Reck, S., Rosenthal, R., Zwahlen, M., Fueglistaler, P., Bolli, M. K., Oertli, D., Widmer, A. F., Marti, W. R. (2009). Surgical Glove Perforation and the Risk of Surgical Site Infection. Arch Surg 144: 553-558 [Abstract] [Full Text]  
  • Stamper, P. D., Alcabasa, R., Aird, D., Babiker, W., Wehrlin, J., Ikpeama, I., Carroll, K. C. (2009). Comparison of a Commercial Real-Time PCR Assay for tcdB Detection to a Cell Culture Cytotoxicity Assay and Toxigenic Culture for Direct Detection of Toxin-Producing Clostridium difficile in Clinical Samples. J. Clin. Microbiol. 47: 373-378 [Abstract] [Full Text]  
  • Cardona, D. M., Rand, K. H. (2008). Evaluation of Repeat Clostridium difficile Enzyme Immunoassay Testing. J. Clin. Microbiol. 46: 3686-3689 [Abstract] [Full Text]  
  • Gilligan, P. H. (2008). Is a Two-Step Glutamate Dehyrogenase Antigen-Cytotoxicity Neutralization Assay Algorithm Superior to the Premier Toxin A and B Enzyme Immunoassay for Laboratory Detection of Clostridium difficile?. J. Clin. Microbiol. 46: 1523-1525 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»