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Journal of Clinical Microbiology, October 2008, p. 3384-3390, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00319-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Proportions of Mycobacterium massiliense and Mycobacterium bolletii Strains among Korean Mycobacterium chelonae-Mycobacterium abscessus Group Isolates{triangledown}

Hee-Youn Kim,1 Yoonwon Kook,2 Yeo-Jun Yun,1 Chan Geun Park,1 Nam Yong Lee,3 Tae Sun Shim,4 Bum-Joon Kim,1 and Yoon-Hoh Kook1*

Department of Microbiology, Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799,1 Life Science and Technology, Underwood International College, Yonsei University, Seoul,2 Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon,3 Division of Pulmonary and Critical Care Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea4

Received 15 February 2008/ Returned for modification 23 April 2008/ Accepted 19 August 2008

Korean isolates of the Mycobacterium chelonae-Mycobacterium abscessus group, which had been isolated from two different hospitals in South Korea, were identified by PCR restriction analysis (PRA) and comparative sequence analysis of 16S rRNA genes, rpoB, and hsp65 to evaluate the proportion of four closely related species (M. chelonae, M. abscessus, M. massiliense, and M. bolletii). Of the 144 rapidly growing mycobacterial strains tested, 127 strains (88.2%) belonged to the M. chelonae-M. abscessus group. In this group, M. chelonae, M. abscessus, M. massiliense, and M. bolletii accounted for 0.8% (n = 1), 51.2% (n = 65), 46.5% (n = 59), and 1.6% (n = 2), respectively. Two isolates which showed discordant results, M. massiliense by rpoB sequence analysis and M. abscessus by hsp65 sequence analysis, were finally identified as M. massiliense based on the additional analysis of sodA and the 16S-23S internal transcribed spacer. M. abscessus group I isolates previously identified by hsp65 PRA were all found to be M. abscessus, whereas group II isolates were further identified as M. massiliense or M. bolletii by sequencing of rpoB and hsp65. Smooth, rough, or mixed colonies of both M. abscessus and M. massiliense isolates were observed. M. massiliense strains that were highly resistant to clarithromycin had a point mutation at the adenine at position 2058 (A2058) or 2059 (A2059) in the peptidyltransferase region of the 23S rRNA gene.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, South Korea. Phone: (82) 2-740-8306. Fax: (82) 2-743-0881. E-mail: yhkook{at}snu.ac.kr

{triangledown} Published ahead of print on 27 August 2008.

Journal of Clinical Microbiology, October 2008, p. 3384-3390, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00319-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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