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Journal of Clinical Microbiology, October 2008, p. 3484-3487, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00837-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of a Multiplex PCR Assay (Bruce-ladder) for Molecular Typing of All Brucella Species, Including the Vaccine Strains{triangledown} ,{dagger}

I. López-Goñi,1* D. García-Yoldi,1 C. M. Marín,2 M. J. de Miguel,2 P. M. Muñoz,2 J. M. Blasco,2 I. Jacques,3,4 M. Grayon,4 A. Cloeckaert,4 A. C. Ferreira,5 R. Cardoso,5 M. I. Corrêa de Sá,5 K. Walravens,6 D. Albert,7 and B. Garin-Bastuji7

Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008 Pamplona, Spain,1 Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 50080 Zaragoza, Spain,2 Institut Universitaire de Technologie, Université François Rabelais, 29 rue du Pont-Volant, 37082 Tours Cedex 2, France,3 INRA, UR1282, Infectiologie Animale et Santé Publique, Centre de Recherche de Tours, Nouzilly 37380, France,4 Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisbon, Portugal,5 Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, CODA CERVA, B-1180 Ukkel, Belgium,6 European Community Reference Laboratory for Brucellosis, Unité Zoonoses Bactériennes, AFSSA, F-94706 Maisons-Alfort Cedex, France7

Received 2 May 2008/ Accepted 24 July 2008

An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

* Corresponding author. Mailing address: Departamento de Microbiología y Parasitología, Universidad de Navarra, c/ Irunlarrea no. 1, 31008 Pamplona, Spain. Phone: (34) 948 425600. Fax: (34) 948 425649. E-mail: ilgoni{at}unav.es

{triangledown} Published ahead of print on 20 August 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, October 2008, p. 3484-3487, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00837-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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