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Journal of Clinical Microbiology, October 2008, p. 3494-3497, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00654-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Two-Year Study Evaluating the Potential Loss of Methicillin Resistance in a Methicillin-Resistant Staphylococcus aureus Culture Collection{triangledown}

William Veguilla,1,{dagger}* K. Kealy Peak,1,{dagger} Vicki A. Luna,1 Jill C. Roberts,1 Carisa R. Davis,1 Andrew C. Cannons,1 Philip Amuso,2 and Jacqueline Cattani1

Center for Biological Defense, College of Public Health, University of South Florida, Tampa, Florida,1 Florida Department of Health, Bureau of Laboratories, Tampa, Florida2

Received 7 April 2008/ Returned for modification 26 June 2008/ Accepted 7 August 2008

A reported loss of mecA prompted us to monitor 360 cryostocked methicillin-resistant Staphylococcus aureus strains for stability. Concurrently, 14 well-characterized strains were stored in a Microbank preservation system and subjected to multiple freeze-thaw events. There were no significant declines in the methicillin-resistant populations with either method over a two-year period.

* Corresponding author. Mailing address: Center for Biological Defense, College of Public Health, University of South Florida, 3602 Spectrum Blvd., Tampa, FL 33612. Phone: (813) 974-1488. Fax: (813) 974-1479. E-mail: wveguill{at}health.usf.edu

{triangledown} Published ahead of print on 20 August 2008.

{dagger} W.V. and K.K.P. contributed equally to this paper.

Journal of Clinical Microbiology, October 2008, p. 3494-3497, Vol. 46, No. 10
0095-1137/08/$08.00+0     doi:10.1128/JCM.00654-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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