This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rôças, I. N.
Right arrow Articles by Siqueira, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rôças, I. N.
Right arrow Articles by Siqueira, J. F., Jr.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2008, p. 3599-3606, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00431-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Root Canal Microbiota of Teeth with Chronic Apical Periodontitis{triangledown} ,{dagger}

I. N. Rôças and J. F. Siqueira Jr.*

Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil

Received 4 March 2008/ Returned for modification 10 March 2008/ Accepted 22 August 2008

Samples from infected root canals of 43 teeth with chronic apical periodontitis were analyzed for the presence and relative levels of 83 oral bacterial species and/or phylotypes using a reverse-capture checkerboard hybridization assay. Associations between the most frequently detected taxa were also recorded. The most prevalent taxa were Olsenella uli (74%), Eikenella corrodens (63%), Porphyromonas endodontalis (56%), Peptostreptococcus anaerobius (54%), and Bacteroidetes oral clone X083 (51%). When prevalence was considered only for bacteria present at levels >105, Bacteroidetes clone X083 was the most frequently isolated bacterium (37%), followed by Parvimonas micra (28%), E. corrodens (23%), and Tannerella forsythia (19%). The number of target taxa per canal was directly proportional to the size of the apical periodontitis lesion, with lesions >10 mm in diameter harboring a mean number of approximately 20 taxa. Several positive associations for the most prevalent taxa were disclosed for the first time and may have important ecological and pathogenic implications. In addition to strengthening the association of several cultivable named species with chronic apical periodontitis, the present findings using a large-scale analysis allowed the inclusion of some newly named species and as-yet-uncultivated phylotypes in the set of candidate pathogens associated with this disease.

* Corresponding author. Mailing address: Faculty of Dentistry, Estácio de Sá University, Rua Alfredo Baltazar da Silveira, 580/Cobertura, Recreio, Rio de Janeiro, RJ 22790-701, Brazil. Phone: 55 21 8874-1022. Fax: 55 21 2199-2204. E-mail: jf_siqueira{at}yahoo.com

{triangledown} Published ahead of print on 3 September 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, November 2008, p. 3599-3606, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00431-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Siqueira, J.F. Jr., Rocas, I.N. (2009). Diversity of Endodontic Microbiota Revisited. JDR 88: 969-981 [Abstract] [Full Text]  
  • Sakamoto, M., Siqueira, J. F. Jr., Rocas, I. N., Benno, Y. (2009). Diversity of Spirochetes in Endodontic Infections. J. Clin. Microbiol. 47: 1352-1357 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»