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Journal of Clinical Microbiology, November 2008, p. 3636-3638, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01308-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Measurement of Persistence of Bordetella pertussis DNA in Nasopharyngeal Secretions during Antibiotic Treatment of Young Children with Pertussis {triangledown}

Philippe Bidet,1 Sandrine Liguori,1 Agathe De Lauzanne,2 Valérie Caro,3 Mathie Lorrot,2 Agnès Carol,1 Albert Faye,2 Nicole Guiso,3 Edouard Bingen,1 and Stéphane Bonacorsi1*

Service de Microbiologie,1 Service de Pédiatrie Générale, Hôpital Robert Debré (AP-HP), Paris 75019,2 Institut Pasteur-Centre National de Référence de la Coqueluche et autres Bordetelloses, Paris 75015, France3

Received 10 July 2008/ Returned for modification 25 August 2008/ Accepted 28 August 2008

We used real-time PCR to examine the persistence of Bordetella pertussis DNA in serial nasopharyngeal aspirates from 22 children treated for pertussis. After 5 days of treatment, PCR was positive for all 21 assessable patients. After 14 and 21 days, PCR was still positive for 83% (10/12) and 66% (4/6) of assessable patients, respectively. One patient was tested 1 month after treatment initiation, and B. pertussis DNA was still detectable. Quantitative analysis showed that the DNA concentration diminished during treatment in all except one case. The PCR cycle threshold at which B. pertussis DNA became detectable increased by a mean of 1.7 cycles per day (range, 0.86 to 3.68 cycles per day). Real-time PCR can thus be used to diagnose pertussis in young children for up to 3 weeks after treatment initiation. Its potential value for assessing the treatment outcome remains to be determined.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Robert-Debré, 48 Boulevard Sérurier, Paris 75395 cedex 19, France. Phone: 33 1 40 03 23 40. Fax: 33 1 40 03 24 50. E-mail: stephane.bonacorsi{at}rdb.aphp.fr

{triangledown} Published ahead of print on 3 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3636-3638, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01308-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Caro, V., Guiso, N., Alberti, C., Liguori, S., Burucoa, C., Couetdic, G., Doucet-Populaire, F., Ferroni, A., Papin-Gibaud, S., Grattard, F., Reglier-Poupet, H., Raymond, J., Soler, C., Bouchet, S., Charreau, S., Couzon, B., Leymarie, I., Tavares, N., Choux, M., Bingen, E., Bonacorsi, S. (2009). Proficiency Program for Real-Time PCR Diagnosis of Bordetella pertussis Infections in French Hospital Laboratories and at the French National Reference Center for Whooping Cough and other Bordetelloses. J. Clin. Microbiol. 47: 3197-3203 [Abstract] [Full Text]  
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