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Journal of Clinical Microbiology, November 2008, p. 3672-3677, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00913-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Kinetics of Epstein-Barr Virus DNA Load in Different Blood Compartments of Pediatric Recipients of T-Cell-Depleted HLA-Haploidentical Stem Cell Transplantation{triangledown}

Fausto Baldanti,1,2* Marta Gatti,1 Milena Furione,1 Stefania Paolucci,1 Carmine Tinelli,3 Patrizia Comoli,4 Pietro Merli,4 and Franco Locatelli4

Servizio di Virologia,1 Laboratori Sperimentali di Ricerca,2 Servizio di Biometria ed Epidemiologia Clinica,3 Clinica di Oncoematologia Pediatrica, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Università degli Studi di Pavia, 27100 Pavia, Italy4

Received 13 May 2008/ Returned for modification 26 July 2008/ Accepted 7 September 2008

Epstein-Barr virus (EBV) DNA levels in whole-blood samples of 54 pediatric patients receiving T-cell-depleted haploidentical hematopoietic stem cell transplantation (HSCT) in 2003 to 2007 were retrospectively compared with EBV DNA loads in peripheral blood mononuclear cells (PBMC). Determination of EBV DNA in whole blood missed 1 of 19 patients (5.2%), who tested positive for EBV DNA in PBMC. The analytical sensitivity of EBV DNA detection in whole-blood samples relative to that in PBMC was 94.7%. Regression analysis showed a significant correlation between DNA levels in PBMC and whole blood (r = 0.81; P < 0.001). Relative to that in PBMC, the appearance of EBV DNA in whole blood was delayed in 9/18 patients (median, 49 days; range, 6 to 226 days), while peak levels and clearance were reached simultaneously. Following peak levels, EBV DNA showed a slower decline in whole blood than in PBMC. In conclusion, (i) EBV DNA levels in PBMC were significantly correlated with those in whole blood; (ii) a differential kinetics of EBV DNA in the two blood compartments was observed; and (iii) monitoring of EBV DNA levels in whole blood appears to be a valuable alternative to PBMC in the follow-up of pediatric recipients of haploidentical T-cell-depleted HSCT.

* Corresponding author. Mailing address: Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy. Phone: 39 0382 502420. Fax: 39 0382 502599. E-mail: f.baldanti{at}smatteo.pv.it

{triangledown} Published ahead of print on 17 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3672-3677, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00913-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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