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Journal of Clinical Microbiology, November 2008, p. 3752-3758, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00377-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Plastic Polymers for Efficient DNA Microarray Hybridization: Application to Microbiological Diagnostics{triangledown}

Zhengshan Zhao,1 Régis Peytavi,1 Gerardo A. Diaz-Quijada,2 Francois J. Picard,1 Ann Huletsky,1 Éric Leblanc,1 Johanne Frenette,1 Guy Boivin,1 Teodor Veres,2 Michel M. Dumoulin,2 and Michel G. Bergeron1*

Centre de Recherche en Infectiologie de l'Université Laval, 2705 Boul. Laurier, Québec, Québec, Canada, G1V 4G2,1 Industrial Materials Institute, National Research Council of Canada, 75 de Mortagne, Boucherville, Québec, Canada, J4B 6Y42

Received 25 February 2008/ Returned for modification 1 July 2008/ Accepted 31 August 2008

Fabrication of microarray devices using traditional glass slides is not easily adaptable to integration into microfluidic systems. There is thus a need for the development of polymeric materials showing a high hybridization signal-to-background ratio, enabling sensitive detection of microbial pathogens. We have developed such plastic supports suitable for highly sensitive DNA microarray hybridizations. The proof of concept of this microarray technology was done through the detection of four human respiratory viruses that were amplified and labeled with a fluorescent dye via a sensitive reverse transcriptase PCR (RT-PCR) assay. The performance of the microarray hybridization with plastic supports made of PMMA [poly(methylmethacrylate)]-VSUVT or Zeonor 1060R was compared to that with high-quality glass slide microarrays by using both passive and microfluidic hybridization systems. Specific hybridization signal-to-background ratios comparable to that obtained with high-quality commercial glass slides were achieved with both polymeric substrates. Microarray hybridizations demonstrated an analytical sensitivity equivalent to approximately 100 viral genome copies per RT-PCR, which is at least 100-fold higher than the sensitivities of previously reported DNA hybridizations on plastic supports. Testing of these plastic polymers using a microfluidic microarray hybridization platform also showed results that were comparable to those with glass supports. In conclusion, PMMA-VSUVT and Zeonor 1060R are both suitable for highly sensitive microarray hybridizations.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie de l'Université Laval, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, 2705 Boul. Laurier, Québec, Québec, Canada, G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Michel.G.Bergeron{at}crchul.ulaval.ca

{triangledown} Published ahead of print on 10 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3752-3758, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00377-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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