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Journal of Clinical Microbiology, November 2008, p. 3833-3835, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01060-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Three Commercial Methods for Rapid Detection of Clostridium difficile Toxins A and B from Fecal Specimens{triangledown}

L. Alcalá,* L. Sánchez-Cambronero, M. P. Catalán, M. Sánchez-Somolinos, M. T. Peláez, M. Marín, and E. Bouza

Hospital General Universitario Gregorio Marañón, Universidad Complutense, Madrid, Spain

Received 4 June 2008/ Returned for modification 21 July 2008/ Accepted 31 August 2008

Three rapid enzyme immunoassays (X/pect Clostridium difficile Toxin A/B test, Wampole Tox A/B Quik Chek, and ImmunoCard Toxins A&B) were compared for the diagnosis of Clostridium difficile infection. Of the 367 stool specimens tested, 102 (27.8%) were positive for toxigenic C. difficile when a combination of direct cytotoxicity assay and cytotoxic culture was used as the gold standard. Sensitivity/specificity values were 49.0%/95.8%, 54.9%/95.5%, and 66.7%/95.1%, respectively. The median times to test five stool specimens were 28, 30, and 24 min, respectively. The ImmunoCard test was the quickest and most sensitive test of the three enzyme immunoassays evaluated.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital General Universitario Gregorio Marañón, Avda. Dr. Esquerdo, 46, 28007 Madrid, Spain. Phone: 34-915868910. Fax: 34-915044906. E-mail: luisalcala{at}efd.net

{triangledown} Published ahead of print on 10 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3833-3835, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01060-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, H., Weintraub, A., Fang, H., Nord, C. E. (2009). Comparison of a Commercial Multiplex Real-Time PCR to the Cell Cytotoxicity Neutralization Assay for Diagnosis of Clostridium difficile Infections. J. Clin. Microbiol. 47: 3729-3731 [Abstract] [Full Text]  
  • Peterson, L. R., Robicsek, A. (2009). Does My Patient Have Clostridium difficile Infection?. ANN INTERN MED 151: 176-179 [Abstract] [Full Text]  


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