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Journal of Clinical Microbiology, November 2008, p. 3839-3841, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00482-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development of Natural Culture Media for Rapid Induction of Fonsecaea pedrosoi Sclerotic Cells In Vitro{triangledown}

Moises Batista da Silva,1 Jorge Pereira da Silva,1,2 Suellen Sirleide Pereira Yamano,1 Ubirajara Imbiriba Salgado,1,3 José Antonio Picanço Diniz,4 and Claudio Guedes Salgado1,5*

Laboratório de Dermato-Imunologia, Universidade do Estado do Pará (UEPA), Universidade Federal do Pará (UFPA), and Unidade de Referência em Dermatologia Sanitária do Estado do Pará Dr. Marcello Candia (MC), Marituba,1 Departamento de Farmácia,2 Departamento de Patologia, UFPA,5 Serviço de Dermatologia, UEPA,3 Laboratório de Microscopia Eletrônica do Instituto Evandro Chagas, Belém, Pará, Brazil4

Received 11 March 2008/ Returned for modification 25 April 2008/ Accepted 10 September 2008

Fonsecaea pedrosoi is the main agent of chromoblastomycosis, a skin disease presenting verrucous lesions, in which round, thick-walled sclerotic cells are found. In vitro induction of sclerotic cells is time-consuming (20 to 45 days) and temperature dependent. We present two new natural media that reduce the sclerotic-cell induction time to only 2 days.

* Corresponding author. Mailing address: Laboratório de Dermato-Imunologia, UEPA/UFPA/MC, Av. João Paulo II, 113, Bairro Dom Aristides, 67200-000, Marituba, Pará, Brazil. Phone and fax: (55) (91) 3256-9097. E-mail: csalgado{at}ufpa.br

{triangledown} Published ahead of print on 17 September 2008.

Journal of Clinical Microbiology, November 2008, p. 3839-3841, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.00482-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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