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Journal of Clinical Microbiology, November 2008, p. 3842-3844, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01155-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Resistance to Macrolides in Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization{triangledown}

Michaela Haas,1 Andreas Essig,1 Edda Bartelt,2 and Sven Poppert3*

Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm,1 Institute for Fish and Fishproducts, Cuxhaven,2 Bernhard Nocht Institute of Tropical Medicine, Hamburg, Germany3

Received 18 June 2008/ Returned for modification 30 July 2008/ Accepted 18 August 2008

The resistance of enteritis-causing Campylobacter strains to erythromycin is an emerging problem. We therefore evaluated fluorescence in situ hybridization (FISH) for the rapid detection of resistance using 74 campylobacter isolates. FISH showed specificity and sensitivity of 100% for the detection of high-level resistance.

* Corresponding author. Mailing address: Abteilung Molekulare Parasitologie, Bernhard Nocht Institut für Tropenmedizin, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany. Phone: 49-40-18 20 93 78. Fax: 49-40-42 81 85 12. E-mail: poppert{at}bni-hamburg.de

{triangledown} Published ahead of print on 27 August 2008.

Journal of Clinical Microbiology, November 2008, p. 3842-3844, Vol. 46, No. 11
0095-1137/08/$08.00+0     doi:10.1128/JCM.01155-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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