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Journal of Clinical Microbiology, December 2008, p. 4029-4033, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01014-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Bacterial and Yeast Species with the Bactec 9120 Automated System with Routine Use of Aerobic, Anaerobic, and Fungal Media{triangledown}

Alfredo Chiarini,1,2* Angelo Palmeri,1 Teresa Amato,2 Rita Immordino,2 Salvatore Distefano,1,2 and Anna Giammanco1,2

Division of Microbiology, Department of Sciences for Health Promotion, University of Palermo,1 A.O.U.P. Paolo Giaccone, Microbiology Laboratory, Palermo, Italy2

Received 28 May 2008/ Returned for modification 9 July 2008/ Accepted 7 October 2008

During the period 2006 and 2007, all blood cultures required by four units at high infective risk and most of those required by other units of the University Hospital of Palermo, Palermo, Italy were performed using a Bactec 9120 automated blood culture system with a complete set of Plus Aerobic/F, Plus Anaerobic/F, and Mycosis IC/F bottles. The aim of the study was to enable the authors to gain firsthand experience of the culture potentialities of the three different media, to obtain information regarding the overall and specific recovery of bacteria and yeasts from blood cultures in the hospital, and to reach a decision as to whether and when to utilize anaerobic and fungal bottles. Although very few bloodstream infections (1.8%) were associated with obligate anaerobes, the traditional routine use of anaerobic bottles was confirmed because of their usefulness, not only in the detection of anaerobes, but also in that of gram-positive cocci and fermentative gram-negative bacilli. In this study, Mycosis IC/F bottles detected 77.4% of all the yeast isolates, 87.0% of yeasts belonging to the species Candida albicans, and 45.7% of nonfermentative gram-negative bacilli resistant to chloramphenicol and tobramycin. In order to improve the diagnosis of fungemia in high-risk patients, the additional routine use of fungal bottles was suggested when, as occurred in the intensive-care unit and in the hematology unit of the University Hospital of Palermo, high percentages of bloodstream infections are associated with yeasts, and/or antibiotic-resistant bacteria and/or multiple bacterial isolates capable of inhibiting yeast growth in aerobic bottles.

* Corresponding author. Mailing address: Department of Sciences for Health Promotion, Via del Vespro 133, 90127 Palermo, Italy. Phone: 39(091)6553660. Fax: 39(091)6553676. E-mail: chiogia{at}unipa.it

{triangledown} Published ahead of print on 15 October 2008.

Journal of Clinical Microbiology, December 2008, p. 4029-4033, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01014-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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