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Journal of Clinical Microbiology, December 2008, p. 4049-4051, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01446-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform{triangledown}

Jason J. Leblanc,1 Janice Pettipas,1 Ross J. Davidson,1,2 Graham A. Tipples,3 Joanne Hiebert,3 and Todd F. Hatchette1,2*

Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada,1 Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada,2 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada3

Received 28 July 2008/ Returned for modification 24 September 2008/ Accepted 9 October 2008

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.

* Corresponding author. Mailing address: Room 404B, MacKenzie Building, Queen Elizabeth II Health Sciences Centre, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada. Phone: (902) 473-6885. Fax: (902) 473-6432. E-mail: todd.hatchette{at}cdha.nshealth.ca

{triangledown} Published ahead of print on 22 October 2008.

Journal of Clinical Microbiology, December 2008, p. 4049-4051, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01446-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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