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Journal of Clinical Microbiology, December 2008, p. 4058-4060, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01411-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Colony Multiplex-Tandem PCR for Rapid, Accurate Identification of Fungal Cultures{triangledown}

Anna Lau,1,2 Tania C. Sorrell,1,2 OkCha Lee,3 Keith Stanley,4 and Catriona Halliday1,3*

Centre for Infectious Diseases and Microbiology,1 Westmead Millennium Institute, University of Sydney,2 Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Sydney West Area Health Service,3 AusDiagnostics Pty. Ltd., 3/36 O'Riordan St., Alexandria, New South Wales, Australia4

Received 23 July 2008/ Returned for modification 21 August 2008/ Accepted 12 October 2008

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales 2145, Australia. Phone: 61-2-9845 6255. Fax: 61-2-9891 5317. E-mail: Catriona.Halliday{at}swahs.health.nsw.gov.au

{triangledown} Published ahead of print on 22 October 2008.

Journal of Clinical Microbiology, December 2008, p. 4058-4060, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01411-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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