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Journal of Clinical Microbiology, December 2008, p. 4095-4098, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01104-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparative Study for Determination of Mycobacterium tuberculosis Susceptibility to First- and Second-Line Antituberculosis Drugs by the Etest Using 7H11, Blood, and Chocolate Agar{triangledown}

Ahmet Yilmaz Coban,1* Kemal Bilgin,1 Meltem Uzun,2 Alper Akgunes,1,3 Anne Yusof,4 and Belma Durupinar1

Ondokuz Mayis University Medical School, Department of Microbiology and Clinical Microbiology, Samsun, Turkey,1 Istanbul University, Istanbul Medical School, Department of Microbiology and Clinical Microbiology, Istanbul, Turkey,2 Chest Disease Hospital, Mycobacteriology Laboratory, Samsun, Turkey,3 AB Biodisk Research Laboratories, Dalvagen 10, 169 56 Solna, Sweden4

Received 10 June 2008/ Returned for modification 2 August 2008/ Accepted 12 October 2008

We investigated the performance of blood and chocolate agar as alternatives to Middlebrook 7H11 agar for testing the susceptibility of Mycobacterium tuberculosis to first-and second-line drugs by the Etest method. A total of 39 strains of M. tuberculosis including 22 multidrug-resistant M. tuberculosis strains and 17 susceptible strains were tested. In conclusion, our results showed that chocolate agar gave insufficient growth, needing up to 21 days of incubation, while results on blood agar were comparable to those on Middlebrook 7H11 agar and can be further explored as an alternative for Etest-based susceptibility testing of M. tuberculosis.

* Corresponding author. Mailing address: Ondokuz Mayis University Medical School, Department of Microbiology and Clinical Microbiology, 55139 Samsun, Turkey. Phone: 90 362 3121919/3526. Fax: 90 362 4576041. E-mail: cobanay2003{at}yahoo.com.tr

{triangledown} Published ahead of print on 22 October 2008.

Journal of Clinical Microbiology, December 2008, p. 4095-4098, Vol. 46, No. 12
0095-1137/08/$08.00+0     doi:10.1128/JCM.01104-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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