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Journal of Clinical Microbiology, February 2008, p. 644-651, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.00801-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid Detection and Molecular Serotyping of Adenovirus by Use of PCR Followed by Electrospray Ionization Mass Spectrometry{triangledown}

Lawrence B. Blyn,1* Thomas A. Hall,1 Brian Libby,1 Raymond Ranken,1 Rangarajan Sampath,1 Karl Rudnick,3 Emily Moradi,3 Anjali Desai,3 David Metzgar,2 Kevin L. Russell,2 Nikki E. Freed,2 Melinda Balansay,2 Michael P. Broderick,2 Miguel A. Osuna,2 Steven A. Hofstadler,1 and David J. Ecker1

Ibis Biosciences, Inc., Carlsbad, California,1 Department of Respiratory Disease Research, Naval Health Research Center, San Diego, California,2 Science Applications International Corporation, San Diego, California3

Received 15 April 2007/ Returned for modification 30 July 2007/ Accepted 30 November 2007

We have developed a PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) assay for the rapid detection, identification, and serotyping of human adenoviruses. The assay employs a high-performance mass spectrometer to "weigh" the amplicons obtained from PCR using primers designed to amplify known human adenoviruses. Masses are converted to base compositions and, by comparison against a database of the genetic sequences, the serotype present in a sample is determined. The performance of the assay was demonstrated with quantified viral standards and environmental and human clinical samples collected from a military training facility. Over 500 samples per day can be analyzed with sensitivities greater than 100 genomes per reaction. This approach can be applied to many other families of infectious agents for rapid and sensitive analysis.

* Corresponding author. Mailing address: Ibis Biosciences, Inc., Carlsbad, CA 92008. Phone: (760) 603-2579. Fax: (760) 603-4653. E-mail: lblyn{at}isisph.com

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 644-651, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.00801-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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