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Journal of Clinical Microbiology, February 2008, p. 685-688, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01808-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Commercial Antisera for Salmonella Serotyping{triangledown}

Kimmi N. Schrader, Ali Fernandez-Castro, Wendy K. W. Cheung, Claudia M. Crandall, and Sharon L. Abbott*

Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Public Health, Richmond, California 94804

Received 11 September 2007/ Returned for modification 5 October 2007/ Accepted 7 December 2007

We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (Tokyo, Japan) somatic and flagellar antisera (using a tube agglutination assay) were 94.0% and 99.2%, respectively, and the specificity was 100% for both sets of sera. Polyvalent O and O1 antiserum sensitivity and specificity were >90%, with the exception of polyvalent O1 antiserum, for which sensitivity was 88.9%. When Denka Seiken flagellar antisera were used in a slide agglutination assay, the sensitivity and accuracy dropped to 88.9% and the specificity fell to 91%. Overall, Denka Seiken commercial antisera performed very well and, together with the comprehensive range of factors available, offer laboratories quality reagents suitable for serotyping strains of salmonellae.

* Corresponding author. Mailing address: Microbial Diseases Laboratory, 850 Marina Bay Parkway, Room E164, Richmond, CA 94804. Phone: (510) 412-3737. Fax: (510) 412-3902. E-mail: Sharon.Abbott{at}cdph.ca.gov

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 685-688, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01808-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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