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Journal of Clinical Microbiology, February 2008, p. 780-784, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01615-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Discrimination of Herpes Simplex Virus Type 2 Strains by Nucleotide Sequence Variations{triangledown}

Hisatoshi Kaneko,1 Takashi Kawana,2 Ken Ishioka,1 Eiko Fukushima,1 and Tatsuo Suzutani1*

Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima 960-1295,1 Department of Obstetrics and Gynecology, University Hospital, Mizonouchi, Teikyo University School of Medicine, Kawasaki 213-8507, Japan2

Received 13 August 2007/ Returned for modification 4 October 2007/ Accepted 20 November 2007

We determined the polymorphous 400-bp regions in UL53, US1, and US4 for the discrimination of herpes simplex virus type 2 (HSV-2) strains. Thirty-six HSV-2 clinical strains could be differentiated into 35 groups using these three regions and into 36 groups by additional analysis of three noncoding regions previously reported as polymorphous.

* Corresponding author. Mailing address: Department of Microbiology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan. Phone: 81-24-547-1158. Fax: 81-24-548-5072. E-mail: suzutani{at}fmu.ac.jp

{triangledown} Published ahead of print on 12 December 2007.

Journal of Clinical Microbiology, February 2008, p. 780-784, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01615-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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