This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhou, L.
Right arrow Articles by Langford, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhou, L.
Right arrow Articles by Langford, P. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2008, p. 800-803, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01787-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains{triangledown}

L. Zhou,1 S. C. P. Jones,1 Ø. Angen,2 J. T. Bossé,1 J. H. E. Nash,3 J. Frey,4 R. Zhou,5 H. C. Chen,5 J. S. Kroll,1 A. N. Rycroft,6 and P. R. Langford1*

Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom,1 National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, DK-1790 Copenhagen V, Denmark,2 Pathogen Genomics Group, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada,3 Institute of Veterinary Bacteriology, University of Berne, Laenggassstrasse 122, 3012 Berne, Switzerland,4 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Shizishan Street 1, Wuhan 430070, People's Republic of China,5 Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, North Mymms, Hertfordshire AL9 7TA, United Kingdom6

Received 7 September 2007/ Returned for modification 5 November 2007/ Accepted 5 December 2007

We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.

* Corresponding author. Mailing address: Department of Paediatrics, Imperial College London, St. Mary's Campus, London W2 1PG, United Kingdom. Phone: 44-(0)20-7594-3359. Fax: 44-(0)20-7594-3984. E-mail: p.langford{at}imperial.ac.uk

{triangledown} Published ahead of print on 19 December 2007.

Journal of Clinical Microbiology, February 2008, p. 800-803, Vol. 46, No. 2
0095-1137/08/$08.00+0     doi:10.1128/JCM.01787-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»