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Journal of Clinical Microbiology, March 2008, p. 1104-1105, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.02259-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparison of Results Obtained with Amplicor HIV-1 DNA PCR Test Version 1.5 Using 100 versus 500 Microliters of Whole Blood{triangledown}

Estelle Piwowar-Manning,1,2 Lebah Lugalia,1,2 Bosco Kafufu,2 and J. Brooks Jackson1,2*

Department of Pathology, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, Maryland 21287,1 Makerere University-Johns Hopkins University Research Collaboration Laboratory, Old Mulago Hill Road, Kampala, Uganda2

Received 21 November 2007/ Returned for modification 19 December 2007/ Accepted 4 January 2008

The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 µl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 µl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.

* Corresponding author. Mailing address: Department of Pathology, Carnegie 415, 600 North Wolfe St., Johns Hopkins Medical Institutions, Baltimore, MD 21287. Phone: (410) 614-4966. Fax: (410) 614-2907. E-mail: bjackso{at}jhmi.edu

{triangledown} Published ahead of print on 16 January 2008.

Journal of Clinical Microbiology, March 2008, p. 1104-1105, Vol. 46, No. 3
0095-1137/08/$08.00+0     doi:10.1128/JCM.02259-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Slogrove, A. L., Cotton, M. F., Esser, M. M. (2009). Severe Infections in HIV-Exposed Uninfected Infants: Clinical Evidence of Immunodeficiency. J Trop Pediatr 0: fmp057v1-fmp057 [Abstract] [Full Text]  


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