This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawatsu, K.
Right arrow Articles by Inoue, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawatsu, K.
Right arrow Articles by Inoue, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2008, p. 1226-1231, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02170-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of Immunochromatographic Assay for Simple and Rapid Detection of Campylobacter jejuni and Campylobacter coli in Human Stool Specimens{triangledown}

Kentaro Kawatsu,* Yuko Kumeda, Masumi Taguchi, Wataru Yamazaki-Matsune, Masashi Kanki, and Kiyoshi Inoue

Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan

Received 9 November 2007/ Returned for modification 4 January 2008/ Accepted 25 January 2008

An immunochromatographic assay (Campy-ICA) using a newly generated single monoclonal antibody against a 15-kDa cell surface protein of Campylobacter jejuni was developed. When cell suspensions of 86 C. jejuni strains and 27 Campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the Campy-ICA, they all yielded positive results. The minimum detectable limits for the C. jejuni strains ranged from 1.8 x 104 to 8.2 x 105 CFU/ml of cell suspension, and those for the C. coli strains ranged from 1.4 x 105 to 4.6 x 106 CFU/ml of cell suspension. All 26 non-Campylobacter species tested yielded negative results with the Campy-ICA. To evaluate the ability of the Campy-ICA to detect C. jejuni and C. coli in human stool specimens, suspensions of 222 stool specimens from patients with acute gastroenteritis were treated with the bacterial protein extraction reagent, and the resulting extracts were tested with the Campy-ICA. The Campy-ICA results showed a sensitivity of 84.8% (28 of 33 specimens) and a specificity of 100% (189 of 189 specimens) compared to the results of isolation of C. jejuni and C. coli from the stool specimens by a bacterial culture test. The Campy-ICA was simple to perform and was able to detect Campylobacter antigen in a fecal extract within 15 min. These results suggest that Campy-ICA testing of fecal extracts may be useful as a simple and rapid adjunct to stool culture for detecting C. jejuni and C. coli in human stool specimens.

* Corresponding author. Mailing address: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 3-69, Nakamichi 1-chome, Higashinari-ku, Osaka 537-0025, Japan. Phone: 81-6-6972-1321, ext. 361. Fax: 81-6-6972-2393. E-mail: kawatu{at}iph.pref.osaka.jp

{triangledown} Published ahead of print on 6 February 2008.

Journal of Clinical Microbiology, April 2008, p. 1226-1231, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02170-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»