This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reijans, M.
Right arrow Articles by Simons, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reijans, M.
Right arrow Articles by Simons, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2008, p. 1232-1240, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02294-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

RespiFinder: a New Multiparameter Test To Differentially Identify Fifteen Respiratory Viruses{triangledown}

Martin Reijans,1* Gijs Dingemans,1,{dagger} Corné H. Klaassen,1 Jacques F. Meis,1 Judith Keijdener,2 Brit Mulders,2 Kimberly Eadie,3 Willem van Leeuwen,3 Alex van Belkum,3 Alphons M. Horrevorts,1 and Guus Simons2

Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands,1 PathoFinder BV, Maastricht, The Netherlands,2 Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands3

Received 29 November 2007/ Returned for modification 2 January 2008/ Accepted 25 January 2008

Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) technology. This assay detects 15 respiratory viruses in one reaction. The MLPA reaction is preceded by a preamplification step which ensures the detection of both RNA and DNA viruses with the same specificity and sensitivity as individual monoplex real-time reverse transcription-PCRs. The RespiFinder assay was validated with 144 clinical samples, and the results of the assay were compared to those of cell culture and a respiratory syncytial virus (RSV)-specific immunochromatography assay (ICA). Compared to the cell culture results, the RespiFinder assay showed specificities and sensitivities of 98.2% and 100%, respectively, for adenovirus; 96.4% and 100%, respectively, for human metapneumovirus; 98.2% and 100%, respectively, for influenza A virus (InfA); 99.1% and 100%, respectively, for parainfluenza virus type 1 (PIV-1); 99.1% and 80%, respectively, for PIV-3; 90.1% and 100%, respectively, for rhinovirus; and 94.6% and 100%, respectively, for RSV. Compared to the results of the RSV-specific ICA, the RespiFinder assay gave a specificity and a sensitivity of 82.4% and 80%, respectively. PIV-2, PIV-4, influenza B virus, InfA H5N1, and coronavirus 229E were not detected in the clinical specimens tested. The use of the RespiFinder assay resulted in an increase in the diagnostic yield compared to that obtained by cell culture (diagnostic yields, 60% and 35.5%, respectively). In conclusion, the RespiFinder assay provides a user-friendly and high-throughput tool for the simultaneous detection of 15 respiratory viruses with excellent overall performance statistics.

* Corresponding author. Present address: PathoFinder B.V., Oxfordlaan 70, 6229 EV Maastricht, The Netherlands. Phone: 31(0)433885852. Fax: 31(0)847469245. E-mail: martin.reijans{at}pathofinder.com

{triangledown} Published ahead of print on 6 February 2008.

{dagger} Present address: PathoFinder B.V., Maastricht, The Netherlands.

Journal of Clinical Microbiology, April 2008, p. 1232-1240, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.02294-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wolffs, P. F. G., Vink, C., Keijdener, J., Habek, B., Reijans, M., Simons, G., Bruggeman, C. A., van den Brule, A. J. C. (2009). Evaluation of MeningoFinder, a Novel Multiplex Ligation-Dependent Probe Amplification Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections. J. Clin. Microbiol. 47: 2620-2622 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»