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Journal of Clinical Microbiology, April 2008, p. 1356-1362, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01785-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Increase of Integration Events and Infection Loads of Human Papillomavirus Type 52 with Lesion Severity from Low-Grade Cervical Lesion to Invasive Cancer{triangledown}

Jo L. K. Cheung,1 T. H. Cheung,2 Julian W. T. Tang,1 and Paul K. S. Chan1,3*

Departments of Microbiology,1 Obstetrics, and Gynaecology,2 School of Public Health, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China3

Received 7 September 2007/ Returned for modification 11 January 2008/ Accepted 1 February 2008

Infection load and the integration of human papillomaviruses (HPV) have been implicated as determinants for oncogenesis, but whether variation among different HPV types exists remains unclear. We investigated 91 women infected with HPV type 52 (HPV-52), a type that is rare worldwide but common in East Asia. The median viral load increased with the severity of the lesion (248 copies/cell equivalent for normal/cervical intraepithelial neoplasia [CIN] grade 1, 402 copies/cell equivalent for CIN 2, 523 copies/cell equivalent for CIN 3, and 1,435 copies/cell equivalent for invasive cancer). The proportion of specimens with integration increased significantly with the severity of the lesion (P < 0.001). The viral load was associated with the physical status of the viral genome, with higher levels for the pure episomal form (P = 0.001). Infection status should be considered when interpreting viral load data for HPV-52, as single infections with this HPV type were found to have marginally higher viral loads than coinfections (P = 0.051). All except one sample had E2 disruption restricted to only a part of the gene. Integration is a critical step in HPV-52-induced carcinogenesis. The profile of E2 disruption was different from that described for HPV-16, with the amino-terminal region being most frequently involved. Selecting the appropriate E2 region for amplification is critical in studying the integration of HPV-52. In summary, the HPV-52 viral load and the integrated proportion increased with the severity of the cervical lesions but had a different pattern than that of HPV-16.

* Corresponding author. Mailing address: Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China. Phone: (852) 2632 3333. Fax: (852) 2647 3227. E-mail: paulkschan{at}cuhk.edu.hk

{triangledown} Published ahead of print on 13 February 2008.

Journal of Clinical Microbiology, April 2008, p. 1356-1362, Vol. 46, No. 4
0095-1137/08/$08.00+0     doi:10.1128/JCM.01785-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cheung, J. L. K., Cheung, T.-H., Ng, C. W. Y., Yu, M. Y., Wong, M. C. S., Siu, S.-S. N., Yim, S.-F., Chan, P. K. S. (2009). Analysis of Human Papillomavirus Type 18 Load and Integration Status from Low-Grade Cervical Lesion to Invasive Cervical Cancer. J. Clin. Microbiol. 47: 287-293 [Abstract] [Full Text]  


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