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Journal of Clinical Microbiology, May 2008, p. 1602-1605, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02483-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Pilot Study of Prevalence of High-Risk Human Papillomavirus Genotypes in Israeli Jewish Women Referred for Colposcopic Examination{triangledown}

Dan Grisaru,1,{dagger}* Boaz Avidor,2,{dagger} Jacob Niv,1 Silvia Marmor,3 Benjamin Almog,1 Cecilia Leibowitz,2 Merav Graidy,2 and Michael Giladi4

Department of Obstetrics and Gynecology,1 Laboratory for Viruses and Molecular Biology, The Bernard Pridan Laboratory for Molecular Biology of Infectious Diseases,2 The Infectious Disease Unit,4 Department of Pathology, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel3

Received 28 December 2007/ Returned for modification 14 February 2008/ Accepted 23 February 2008

The objective of the present study was to determine the prevalence of high-risk (HR) human papillomavirus (HPV) genotypes in a group of Israeli Jewish women referred for colposcopic examination. Scrape specimens were prospectively collected from 84 women referred for colposcopic examination. All the women underwent Papanicolaou (Pap) smears and colposcopies, and some also underwent cervical or loop electrosurgical excision procedure biopsy. HR HPV was detected in scrape specimens (Amplicor HPV test; Roche Molecular Systems), and the individual genotypes in these specimens were identified (HPV GenoArray test kit; Hybribio Ltd., Hong Kong). Forty-one (49%) specimens were positive by the Amplicor HPV test. Sixty-four samples (41 positive and 23 negative by the Amplicor HPV test) were also assayed by use of the HPV GenoArray kit. The overall level of agreement between the two assays was 93.8% (Cohen's kappa = 0.98). HR genotypes were found in 37/41 (90%) HPV-positive samples. The prevalences of the HR HPV genotypes in the 37 HPV-positive samples were 41% of patients for HPV type 16 (HPV-16), 22% for HPV-39, 19% for HPV-52, and 14% for HPV-18. Forty-one percent of these patients were infected with a single HR genotype, whereas 59% were infected with mixtures of HR genotypes. The presence of a relatively high percentage of HPV types 39 and 52 and the relatively high incidence of infections with mixtures of genotypes may be one of the reasons for the low rate of conversion from high-grade squamous intraepithelial lesions to invasive carcinoma in Israeli women. Larger and more comprehensive studies are warranted to investigate this issue in greater detail.

* Corresponding author. Mailing address: Gynecologic Oncology Service, Department of Obstetrics & Gynecology, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel. Phone: 972-3-9625622. Fax: 972-3-9625670. E-mail: grisaro{at}post.tau.ac.il

{triangledown} Published ahead of print on 5 March 2008.

{dagger} D. Grisaru and B. Avidor contributed equally to the paper.

Journal of Clinical Microbiology, May 2008, p. 1602-1605, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.02483-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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