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Journal of Clinical Microbiology, May 2008, p. 1861-1866, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00109-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

PCR Method To Identify Salmonella enterica Serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever{triangledown}

Haim Levy,1,2 Souleymane Diallo,3 Sharon M. Tennant,1 Sofie Livio,1 Samba O. Sow,3 Milagritos Tapia,1,3 Patricia I. Fields,4 Matthew Mikoleit,4 Boubou Tamboura,3 Karen L. Kotloff,1 Rosanna Lagos,5 James P. Nataro,1 James E. Galen,1 and Myron M. Levine1*

Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201,1 Israel Institute for Biological Research, Ness Ziona, Israel,2 Centre pour le Développement des Vaccins, Bamako, Mali,3 National Salmonella Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,4 Centro para Vacunas en Desarrollo, Hospital de Niños Roberto del Rio, Servicio de Salud Metropolitano Norte, Santiago, Chile5

Received 18 January 2008/ Returned for modification 3 March 2008/ Accepted 11 March 2008

PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201. Phone: (410) 706-7588. Fax: (410) 706-6205. E-mail: mlevine{at}medicine.umaryland.edu

{triangledown} Published ahead of print on 26 March 2008.

Journal of Clinical Microbiology, May 2008, p. 1861-1866, Vol. 46, No. 5
0095-1137/08/$08.00+0     doi:10.1128/JCM.00109-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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