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Journal of Clinical Microbiology, June 2008, p. 2074-2077, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01858-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens{triangledown}

Robert Benson,1 Maria L. Tondella,1 Julu Bhatnagar,1 Maria da Glória S. Carvalho,1 Jacquelyn S. Sampson,1 Deborah F. Talkington,1 Anne M. Whitney,1 Elizabeth Mothershed,1 Lesley McGee,2 George Carlone,1 Vondguraus McClee,1 Jeannette Guarner,1,2 Sherif Zaki,1 Surang Dejsiri,3 K. Cronin,4 Jian Han,4 and Barry S. Fields1*

CDC, Atlanta, Georgia,1 Emory University, Atlanta, Georgia,2 National Institute of Health, Thailand Ministry of Public Health, Bangkok, Thailand,3 Genaco Biomedical Products, Inc., Huntsville, Alabama4

Received 18 September 2007/ Returned for modification 7 December 2007/ Accepted 26 March 2008

The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.

* Corresponding author. Mailing address: CDC, Mail Stop G03, 1600 Clifton Road, Atlanta, GA 30333. Phone: (404) 639-3395. Fax: (404) 718-1855. E-mail: bfields{at}cdc.gov

{triangledown} Published ahead of print on 9 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2074-2077, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.01858-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rogers, G. B., Carroll, M. P., Bruce, K. D. (2009). Studying bacterial infections through culture-independent approaches. J Med Microbiol 58: 1401-1418 [Abstract] [Full Text]  


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