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Journal of Clinical Microbiology, June 2008, p. 2078-2080, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02179-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of New Commercial Real-Time PCR Quantification Assay for Prenatal Diagnosis of Cytomegalovirus Congenital Infection{triangledown}

Aurélie Ducroux,1 Samira Cherid,1 Alexandra Benachi,2 Yves Ville,3 and Marianne Leruez-Ville1*

Laboratoire de Virologie, Centre Nationale de Référence du Cytomégalovirus, Hôpital Necker-Enfants-malades, Assistance Publique-Hôpitaux de Paris, Université Paris-Descartes, EA 36-20, 149 rue de Sèvres, 75015 Paris, France,1 Service de Gynécologie-Obstétrique, Assistance Publique-Hôpitaux de Paris, Hôpital Necker-Enfants-malades, Assistance Publique-Hôpitaux de Paris, Université Paris-Descartes, 149 rue de Sèvres, 75015 Paris, France,2 Service de Gynécologie-Obstétrique, Hôpital de Poissy-Saint Germain en Laye, Université Saint-Quentin en Yvelines, 10 rue du Champ Gaillard, 78000 Poissy, France3

Received 10 November 2007/ Returned for modification 12 March 2008/ Accepted 9 April 2008

A new commercial real-time human cytomegalovirus (HCMV) PCR kit was evaluated after automated DNA extraction of 153 amniotic fluids in parallel with an in-house real-time PCR assay. The commercial kit displayed 100% sensitivity/specificity compared to the "in-house" assay and was suitable for prenatal diagnosis of HCMV congenital infection.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Necker-Enfants-malades, 149 rue de Sèvres, 75015 Paris, France. Phone: 33 1 44 49 49 62. Fax: 33 1 44 49 49 60. E-mail: marianne.leruez{at}nck.aphp.fr

{triangledown} Published ahead of print on 16 April 2008.

Journal of Clinical Microbiology, June 2008, p. 2078-2080, Vol. 46, No. 6
0095-1137/08/$08.00+0     doi:10.1128/JCM.02179-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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