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Journal of Clinical Microbiology, July 2008, p. 2155-2159, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00493-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Panel for Antifungal Susceptibility Testing of the Echinocandins Anidulafungin, Caspofungin, and Micafungin{triangledown}

M. A. Pfaller,1* V. Chaturvedi,2 D. J. Diekema,1 M. A. Ghannoum,3 N. M. Holliday,4 S. B. Killian,4 C. C. Knapp,4 S. A. Messer,1 A. Miskov,4 and R. Ramani2

University of Iowa College of Medicine, Iowa City, Iowa,1 New York State Department of Health, Albany, New York,2 Case Western Reserve University, Cleveland, Ohio,3 Trek Diagnostic Systems, Cleveland, Ohio4

Received 12 March 2008/ Returned for modification 25 April 2008/ Accepted 29 April 2008

A commercially prepared, dried colorimetric microdilution panel (Sensititre YeastOne Trek Diagnostic Systems, Cleveland, OH) was compared in three different laboratories with the Clinical and Laboratory Standards Institute (CLSI) reference microdilution method by testing 2 quality control strains, 25 reproducibility strains, and 404 isolates of Candida spp. against anidulafungin, caspofungin, and micafungin. Reference MIC endpoints and YeastOne colorimetric endpoints were read after 24 h of incubation. YeastOne endpoints were determined to be the lowest concentration at which the color in the well changed from red (positive, indicating growth) to blue (negative, indicating no growth). Excellent essential agreement (within 2 dilutions) between the reference and colorimetric MICs was observed. Overall agreement was 100% for all three agents. Categorical agreement ranged from 99.3% (anidulafungin) to 100% (caspofungin, micafungin) and interlaboratory reproducibility was 99%. The YeastOne colorimetric method appears to be comparable to the CLSI reference method for testing the susceptibility of Candida spp. to the echinocandins anidulafungin, caspofungin, and micafungin.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 356-8615. Fax: (319) 356-4916. E-mail: michael-pfaller{at}uiowa.edu

{triangledown} Published ahead of print on 7 May 2008.

Journal of Clinical Microbiology, July 2008, p. 2155-2159, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00493-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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