This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, G. Q.
Right arrow Articles by Tang, L. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, G. Q.
Right arrow Articles by Tang, L. H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2008, p. 2167-2174, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00081-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multiplex PCR and Oligonucleotide Microarray for Detection of Single-Nucleotide Polymorphisms Associated with Plasmodium falciparum Drug Resistance{triangledown} ,{dagger}

Guo Qing Zhang,1 Ya Yi Guan,1 Hai Hui Sheng,2 Bin Zheng,1 Song Wu,1 Hua Sheng Xiao,2 and Lin Hua Tang1*

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, and Filariasis, 207 Rui Jin Er Road, Shanghai 200025, China,1 National Engineering Center for Biochip at Shanghai, 151 Li Bing Road, Pudong, Shanghai 201203, China2

Received 31 December 2007/ Returned for modification 14 March 2008/ Accepted 17 April 2008

Drug resistance in Plasmodium falciparum is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucleotide microarray method was developed for the detection of these SNPs in genes encoding chloroquine resistance transporter (Pfcrt), multidrug resistance 1 (Pfmdr1), dihydrofolate reductase (Pfdhfr), dihydropteroate synthetase (Pfdhps), and ATPase 6 (PfATPase6) of P. falciparum. The results show that DNA microarray technology, combined with mPCR, is a promising and time-saving tool that supports conventional detection methods, allowing sensitive, accurate, simultaneous analysis of the SNPs associated with drug resistance in P. falciparum.

* Corresponding author. Mailing address: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, 207 Rui Jin Er Road, Shanghai 200025, China. Phone: 86-21-64373359. Fax: 86-21-64332670. E-mail: ipd_sh{at}163.com

{triangledown} Published ahead of print on 30 April 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2008, p. 2167-2174, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00081-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»