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Journal of Clinical Microbiology, July 2008, p. 2189-2194, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00398-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

MDCK-SIAT1 Cells Show Improved Isolation Rates for Recent Human Influenza Viruses Compared to Conventional MDCK Cells{triangledown} ,{dagger}

Ding Yuan Oh,1,2 Ian G. Barr,1,2 Jenny A. Mosse,2 and Karen L. Laurie1*

WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Melbourne, Australia 3052,1 School of Applied Sciences and Engineering, Monash University Gippsland Campus, Churchill, Victoria, Australia 38422

Received 27 February 2008/ Returned for modification 21 April 2008/ Accepted 2 May 2008

The ability to isolate and propagate influenza virus is an essential tool for the yearly surveillance of circulating virus strains and to ensure accurate clinical diagnosis for appropriate treatment. The suitability of MDCK-SIAT1 cells, engineered to express increased levels of {alpha}-2,6-linked sialic acid receptors, as an alternative to conventional MDCK cells for isolation of circulating influenza virus was assessed. A greater number of influenza A (H1N1 and H3N2) and B viruses from stored human clinical specimens collected between 2005 and 2007 were isolated following inoculation in MDCK-SIAT1 cells than in MDCK cells. In addition, a higher titer of virus was recovered following culture in MDCK-SIAT1 cells. All A(H1N1) viruses recovered from MDCK-SIAT1 cells were able to agglutinate both turkey and guinea pig red blood cells (RBC), while half of the A(H3N2) viruses recovered after passage in MDCK-SIAT1 cells lost the ability to agglutinate turkey RBC. Importantly, the HA-1 domain of the hemagglutinin gene was genetically stable after passaging in MDCK-SIAT1 cells, a feature not always seen following MDCK cell or embryonated chicken egg passage of human influenza virus. These data indicate that the MDCK-SIAT1 cell line is superior to conventional MDCK cells for isolation of human influenza virus from clinical specimens and may be used routinely for the isolation and propagation of current human influenza viruses for surveillance, diagnostic, and research purposes.

* Corresponding author. Mailing address: WHO Collaborating Centre for Reference and Research on Influenza, 45 Poplar Road, Parkville, Melbourne, Australia 3052. Phone: 61-3-9389-1340. Fax: 61-3-9389-1881. E-mail: karen.laurie{at}influenzacentre.org

{triangledown} Published ahead of print on 14 May 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2008, p. 2189-2194, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00398-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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