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Journal of Clinical Microbiology, July 2008, p. 2384-2388, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00051-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of Streptococcus pneumoniae in Purulent Sputum Samples by Use of a Novel Real-Time Multiplex PCR Assay{triangledown} ,{dagger}

Kazuko Y. Fukushima,1 Katsunori Yanagihara,1* Yoichi Hirakata,2 Kazuyuki Sugahara,1 Yoshitomo Morinaga,1,2 Shigeru Kohno,2 and Shimeru Kamihira1

Department of Laboratory Medicine,1 Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan2

Received 10 January 2008/ Returned for modification 3 March 2008/ Accepted 29 April 2008

We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan. Phone: 81-95-849-7418. Fax: 81-95-849-7257. E-mail: kyana-ngs{at}umin.ac.jp

{triangledown} Published ahead of print on 7 May 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2008, p. 2384-2388, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00051-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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