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Journal of Clinical Microbiology, July 2008, p. 2399-2401, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00024-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Quadruplex PCR for Simultaneous Detection of Serotype, Biotype, Toxigenic Potential, and Central Regulating Factor of Vibrio cholerae{triangledown}

Hemant Kumar Khuntia, Bibhuti Bhusan Pal, and Guru Prasada Chhotray*

Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Pin-751023, Orissa, India

Received 7 January 2008/ Returned for modification 11 February 2008/ Accepted 30 April 2008

A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.

* Corresponding author. Mailing address: Pathology and Microbiology Division, Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Pin-751023, Orissa, India. Phone and fax: 91-674-2301320. E-mail: rmrcpath{at}yahoo.co.in

{triangledown} Published ahead of print on 7 May 2008.

Journal of Clinical Microbiology, July 2008, p. 2399-2401, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00024-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, J., Zhu, Y., Wen, H., Zhang, J., Huang, S., Niu, J., Li, Q. (2009). Quadruplex Real-Time PCR Assay for Detection and Identification of Vibrio cholerae O1 and O139 Strains and Determination of Their Toxigenic Potential. Appl. Environ. Microbiol. 75: 6981-6985 [Abstract] [Full Text]  
  • Grim, C. J., Zo, Y.-G., Hasan, N. A., Ali, A., Chowdhury, W. B., Islam, A., Rashid, M. H., Alam, M., Morris, J. G. Jr., Huq, A., Colwell, R. R. (2009). RNA Colony Blot Hybridization Method for Enumeration of Culturable Vibrio cholerae and Vibrio mimicus Bacteria. Appl. Environ. Microbiol. 75: 5439-5444 [Abstract] [Full Text]  


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